3×3 light grid 106 Search Results


106 bj  (ATCC)
96
ATCC 106 bj
Figure 3. Adipose-derived stem cell (ASC) effect on primary Cal-27 xenografts. 3 × <t>106</t> human Cal-27/GFP cells were bilaterally injected subcutaneously into the flanks of 5 female NUDE mice (n = 8-10 tumors/group) without or with 3 × 106 human ASC/ RFP cells or 3 × 106 <t>BJ-5ta</t> human fibroblasts. (A) Tumor volume was monitored weekly by caliper measurement. *P < .05 for dif- ference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + ASC/RFP tumors; and P < .05 for difference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + BJ-5ta fibroblasts tumors. Representative tumors were removed at 6 weeks from the Cal-27/GFP group (B-E) and the Cal-27/GFP + ASC/RFP group (F-I). Tumors were visualized by light microscopy (B, F), GFP (C, G), RFP (D, H), and a merge of GFP and RFP (E, I) using a dissecting fluorescent microscope. Representative paraffin-embedded tumor sections were prepared for hematoxylin and eosin (H & E) staining for Cal-27/GFP ( J) and Cal-27/GFP + ASC/RFP (N) tumors. Representative frozen sections of tumors were stained with DAPI (blue) for Cal-27/GFP (K-M) and Cal-27/GFP + ASC/RFP (O-Q) tumors. These tumor sections were prepared for fluorescence microscopy for GFP (K, O) and RFP (L, P) or merge of GFP and RFP (M, Q).
106 Bj, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pm26063833-83-12-16?v=ATCC
Average 96 stars, based on 1 article reviews
106 bj - by Bioz Stars, 2026-08
96/100 stars
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goat anti chicken light chain antibodies  (Bethyl)
93
Bethyl goat anti chicken light chain antibodies
Figure 3. Adipose-derived stem cell (ASC) effect on primary Cal-27 xenografts. 3 × <t>106</t> human Cal-27/GFP cells were bilaterally injected subcutaneously into the flanks of 5 female NUDE mice (n = 8-10 tumors/group) without or with 3 × 106 human ASC/ RFP cells or 3 × 106 <t>BJ-5ta</t> human fibroblasts. (A) Tumor volume was monitored weekly by caliper measurement. *P < .05 for dif- ference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + ASC/RFP tumors; and P < .05 for difference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + BJ-5ta fibroblasts tumors. Representative tumors were removed at 6 weeks from the Cal-27/GFP group (B-E) and the Cal-27/GFP + ASC/RFP group (F-I). Tumors were visualized by light microscopy (B, F), GFP (C, G), RFP (D, H), and a merge of GFP and RFP (E, I) using a dissecting fluorescent microscope. Representative paraffin-embedded tumor sections were prepared for hematoxylin and eosin (H & E) staining for Cal-27/GFP ( J) and Cal-27/GFP + ASC/RFP (N) tumors. Representative frozen sections of tumors were stained with DAPI (blue) for Cal-27/GFP (K-M) and Cal-27/GFP + ASC/RFP (O-Q) tumors. These tumor sections were prepared for fluorescence microscopy for GFP (K, O) and RFP (L, P) or merge of GFP and RFP (M, Q).
Goat Anti Chicken Light Chain Antibodies, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/us10766951-274-1-7?v=Bethyl
Average 93 stars, based on 1 article reviews
goat anti chicken light chain antibodies - by Bioz Stars, 2026-08
93/100 stars
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mitomycin c treated splenocytes  (Thermo Fisher)
96
Thermo Fisher mitomycin c treated splenocytes
Figure 3. Adipose-derived stem cell (ASC) effect on primary Cal-27 xenografts. 3 × <t>106</t> human Cal-27/GFP cells were bilaterally injected subcutaneously into the flanks of 5 female NUDE mice (n = 8-10 tumors/group) without or with 3 × 106 human ASC/ RFP cells or 3 × 106 <t>BJ-5ta</t> human fibroblasts. (A) Tumor volume was monitored weekly by caliper measurement. *P < .05 for dif- ference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + ASC/RFP tumors; and P < .05 for difference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + BJ-5ta fibroblasts tumors. Representative tumors were removed at 6 weeks from the Cal-27/GFP group (B-E) and the Cal-27/GFP + ASC/RFP group (F-I). Tumors were visualized by light microscopy (B, F), GFP (C, G), RFP (D, H), and a merge of GFP and RFP (E, I) using a dissecting fluorescent microscope. Representative paraffin-embedded tumor sections were prepared for hematoxylin and eosin (H & E) staining for Cal-27/GFP ( J) and Cal-27/GFP + ASC/RFP (N) tumors. Representative frozen sections of tumors were stained with DAPI (blue) for Cal-27/GFP (K-M) and Cal-27/GFP + ASC/RFP (O-Q) tumors. These tumor sections were prepared for fluorescence microscopy for GFP (K, O) and RFP (L, P) or merge of GFP and RFP (M, Q).
Mitomycin C Treated Splenocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pm28954887-48-20-48?v=Thermo+Fisher
Average 96 stars, based on 1 article reviews
mitomycin c treated splenocytes - by Bioz Stars, 2026-08
96/100 stars
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isotype mouse igg2a control antibody  (Miltenyi Biotec)
95
Miltenyi Biotec isotype mouse igg2a control antibody
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Isotype Mouse Igg2a Control Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pmc09369378-177-51-56?v=Miltenyi+Biotec
Average 95 stars, based on 1 article reviews
isotype mouse igg2a control antibody - by Bioz Stars, 2026-08
95/100 stars
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light grid 106  (Staples)
86
Staples light grid 106
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Light Grid 106, supplied by Staples, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/us12402227-84-33-37?v=Staples
Average 86 stars, based on 1 article reviews
light grid 106 - by Bioz Stars, 2026-08
86/100 stars
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1.5t vision scanner  (Siemens AG)
90
Siemens AG 1.5t vision scanner
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
1.5t Vision Scanner, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pm11675642-120-2-2?v=Siemens+AG
Average 90 stars, based on 1 article reviews
1.5t vision scanner - by Bioz Stars, 2026-08
90/100 stars
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lens  (Nikon)
99
Nikon lens
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Lens, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pm23844052-124-33-34?v=Nikon
Average 99 stars, based on 1 article reviews
lens - by Bioz Stars, 2026-08
99/100 stars
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smartpls version 3.3.3  (SmartPLS GmbH)
90
SmartPLS GmbH smartpls version 3.3.3
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Smartpls Version 3.3.3, supplied by SmartPLS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/10__31130_slash_jst___ud2021___444-118-22-21?v=SmartPLS+GmbH
Average 90 stars, based on 1 article reviews
smartpls version 3.3.3 - by Bioz Stars, 2026-08
90/100 stars
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ver 3.3.3  (SmartPLS GmbH)
90
SmartPLS GmbH ver 3.3.3
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Ver 3.3.3, supplied by SmartPLS GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pmc10123211-540-22-21?v=SmartPLS+GmbH
Average 90 stars, based on 1 article reviews
ver 3.3.3 - by Bioz Stars, 2026-08
90/100 stars
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rstudio version 3.3.3  (RStudio)
90
RStudio rstudio version 3.3.3
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
Rstudio Version 3.3.3, supplied by RStudio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pmc07047643-166-25-24?v=RStudio
Average 90 stars, based on 1 article reviews
rstudio version 3.3.3 - by Bioz Stars, 2026-08
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1,1',3,3,3',3'-hexamethylindodicarbocyanine cy5-dm  (OKeanos Tech Co Ltd)
90
OKeanos Tech Co Ltd 1,1',3,3,3',3'-hexamethylindodicarbocyanine cy5-dm
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
1,1',3,3,3',3' Hexamethylindodicarbocyanine Cy5 Dm, supplied by OKeanos Tech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pmc11199968__ADVS___11___2309517___s001-69-0-5?v=OKeanos+Tech+Co+Ltd
Average 90 stars, based on 1 article reviews
1,1',3,3,3',3'-hexamethylindodicarbocyanine cy5-dm - by Bioz Stars, 2026-08
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1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (dii)  (AnaSpec)
90
AnaSpec 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (dii)
Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse <t>IgG</t> antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.
1,1’ Dioctadecyl 3,3,3’,3’ Tetramethylindocarbocyanine Perchlorate (Dii), supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3%C3%973+light+grid+106/pmc04642858-332-9-12?v=AnaSpec
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1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (dii) - by Bioz Stars, 2026-08
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Image Search Results


Figure 3. Adipose-derived stem cell (ASC) effect on primary Cal-27 xenografts. 3 × 106 human Cal-27/GFP cells were bilaterally injected subcutaneously into the flanks of 5 female NUDE mice (n = 8-10 tumors/group) without or with 3 × 106 human ASC/ RFP cells or 3 × 106 BJ-5ta human fibroblasts. (A) Tumor volume was monitored weekly by caliper measurement. *P < .05 for dif- ference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + ASC/RFP tumors; and P < .05 for difference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + BJ-5ta fibroblasts tumors. Representative tumors were removed at 6 weeks from the Cal-27/GFP group (B-E) and the Cal-27/GFP + ASC/RFP group (F-I). Tumors were visualized by light microscopy (B, F), GFP (C, G), RFP (D, H), and a merge of GFP and RFP (E, I) using a dissecting fluorescent microscope. Representative paraffin-embedded tumor sections were prepared for hematoxylin and eosin (H & E) staining for Cal-27/GFP ( J) and Cal-27/GFP + ASC/RFP (N) tumors. Representative frozen sections of tumors were stained with DAPI (blue) for Cal-27/GFP (K-M) and Cal-27/GFP + ASC/RFP (O-Q) tumors. These tumor sections were prepared for fluorescence microscopy for GFP (K, O) and RFP (L, P) or merge of GFP and RFP (M, Q).

Journal: Aesthetic surgery journal

Article Title: Human Adipose Tissue-Derived Stromal/Stem Cells Promote Migration and Early Metastasis of Head and Neck Cancer Xenografts.

doi: 10.1093/asj/sjv090

Figure Lengend Snippet: Figure 3. Adipose-derived stem cell (ASC) effect on primary Cal-27 xenografts. 3 × 106 human Cal-27/GFP cells were bilaterally injected subcutaneously into the flanks of 5 female NUDE mice (n = 8-10 tumors/group) without or with 3 × 106 human ASC/ RFP cells or 3 × 106 BJ-5ta human fibroblasts. (A) Tumor volume was monitored weekly by caliper measurement. *P < .05 for dif- ference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + ASC/RFP tumors; and P < .05 for difference in tumor volume between Cal-27/GFP-alone tumors and Cal-27/GFP + BJ-5ta fibroblasts tumors. Representative tumors were removed at 6 weeks from the Cal-27/GFP group (B-E) and the Cal-27/GFP + ASC/RFP group (F-I). Tumors were visualized by light microscopy (B, F), GFP (C, G), RFP (D, H), and a merge of GFP and RFP (E, I) using a dissecting fluorescent microscope. Representative paraffin-embedded tumor sections were prepared for hematoxylin and eosin (H & E) staining for Cal-27/GFP ( J) and Cal-27/GFP + ASC/RFP (N) tumors. Representative frozen sections of tumors were stained with DAPI (blue) for Cal-27/GFP (K-M) and Cal-27/GFP + ASC/RFP (O-Q) tumors. These tumor sections were prepared for fluorescence microscopy for GFP (K, O) and RFP (L, P) or merge of GFP and RFP (M, Q).

Article Snippet: Control injections were injection of 3× 106 ASC/RFP cells alone or 3× 106 BJ-5ta human fibroblasts (ATCC® CRL-4001TM)+3× 106 Cal-27/GFP cells.

Techniques: Derivative Assay, Injection, Light Microscopy, Microscopy, Staining, Fluorescence

Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse IgG antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Phage Display Biopanning Strategies for the Selection of Anti-Cell Surface Receptor Antibodies

doi: 10.3390/ijms23158470

Figure Lengend Snippet: Binding analysis of STRE and CBA biopanning derived phage pools and individual phage clones. ( A ) Polyclonal phage ELISA against the recombinant antigens mβc (mu-sAIC2B-His, positive) and mβ IL-3 (mu-sAIC2A-His, negative). Phage pools from all three CBA (cR1-cR3) and STRE (sR1-sR3) rounds were compared to the naïve Jones-Mahler (JM) library. Binding was assessed in duplicates by immobilizing the recombinant proteins on ELISA plates followed by incubation with successive 10-fold dilutions of phage particles. ( B ) Polyclonal flow cytometry analysis of the original phage library and subsequent biopanning pools against transiently expressed mβc-GFP on the surface of CHO-XL99 cells. Protein expression was monitored using the intracellular GFP ( x -axis), while phage binding was determined using a primary mouse anti-M13 antibody in combination with a secondary anti-mouse IgG antibody conjugated to DyLight650 ( y -axis). ( C ) Monoclonal phage ELISA of selected purified phage clones that exceeded an A 450 reading of 0.6 in a preliminary screen . Adipose fatty acid binding protein (AFBP) was used as a non-specific His-tagged negative control. Prefixes ‘c’ and ‘s’ denote clones derived from CBA and STRE biopanning methods, respectively. Clones with no prefix were identified in both biopanning campaigns.

Article Snippet: PE-conjugated rat anti-mouse CD131 (mβc) antibody (BD Pharmingen, cat# 559920, RRID: AB_397374); Mouse anti-M13 antibody (SinoBiological, Beijing, China; cat# 11973-MM05T, RRID: AB_2857926); HRP conjugated goat anti-mouse IgG antibody (BioRad, South Granville, NSW, Australia; cat# 1706516, RRID: AB_11125547); DyLight 650 conjugated goat anti-mouse IgG antibody (Abcam, Melbourne, VIC, Australia; cat# 96874, RRID:AB_10679531); Isotype mouse IgG2a control antibody (Miltenyi Biotec, Macquarie Park, NSW, Australia; cat# 130-106-546, RRID:AB_2661589); PE-conjugated mouse anti-STAT5 (pY694) antibody (BD Biosciences, Macquarie Park, NSW, Australia; cat# 612567, RRID:AB_399858); PE-conjugated rat anti-mouse CD123 (IL-3Rα) antibody (eBioscience, San Diego, CA, USA; cat# 12-1231-82, RRID:AB_465839); AF488 conjugated rat anti-mouse β IL-3 antibody (R&D Systems, Minneapolis, MN, USA; cat# FAB5492G, RRID: RRID:AB_2905558); Rat anti-mouse CD131 (mβc) antibody (BD Biosciences, cat# 740050, RRID:AB_2739817).

Techniques: Binding Assay, Derivative Assay, Clone Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Incubation, Flow Cytometry, Expressing, Purification, Negative Control

Analytical size exclusion chromatography (SEC) of purified anti-mβc mAbs. Chromatograms show the elution profile of each mAb (blue) overlayed on a standard run of proteins with known molecular weights ( M W ) (grey). ( A ) All 30 candidates eluted in a single peak indicative of a homogenous species of molecules in solution. However, six antibodies (cA5, cC4, cG8, sC3, sC9, and sE7) exhibited longer retention times than expected for a full-length IgG molecule when analyzed on a silica-based column. ( B ) These clones were further assessed on a Superdex 200 column coupled to a MALS detector for accurate M W determination. Retention times for all antibodies except sC3 were comparable to the IgG protein in the standard (peak B), and M W s corresponded with theoretically calculated values. Standard proteins: A—thyroglobulin (670 kDa), B—IgG (158 kDa), C—ovalbumin (44 kDa), D—myoglobin (17 kDa), E—vitamin E12 (1.35 kDa).

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Phage Display Biopanning Strategies for the Selection of Anti-Cell Surface Receptor Antibodies

doi: 10.3390/ijms23158470

Figure Lengend Snippet: Analytical size exclusion chromatography (SEC) of purified anti-mβc mAbs. Chromatograms show the elution profile of each mAb (blue) overlayed on a standard run of proteins with known molecular weights ( M W ) (grey). ( A ) All 30 candidates eluted in a single peak indicative of a homogenous species of molecules in solution. However, six antibodies (cA5, cC4, cG8, sC3, sC9, and sE7) exhibited longer retention times than expected for a full-length IgG molecule when analyzed on a silica-based column. ( B ) These clones were further assessed on a Superdex 200 column coupled to a MALS detector for accurate M W determination. Retention times for all antibodies except sC3 were comparable to the IgG protein in the standard (peak B), and M W s corresponded with theoretically calculated values. Standard proteins: A—thyroglobulin (670 kDa), B—IgG (158 kDa), C—ovalbumin (44 kDa), D—myoglobin (17 kDa), E—vitamin E12 (1.35 kDa).

Article Snippet: PE-conjugated rat anti-mouse CD131 (mβc) antibody (BD Pharmingen, cat# 559920, RRID: AB_397374); Mouse anti-M13 antibody (SinoBiological, Beijing, China; cat# 11973-MM05T, RRID: AB_2857926); HRP conjugated goat anti-mouse IgG antibody (BioRad, South Granville, NSW, Australia; cat# 1706516, RRID: AB_11125547); DyLight 650 conjugated goat anti-mouse IgG antibody (Abcam, Melbourne, VIC, Australia; cat# 96874, RRID:AB_10679531); Isotype mouse IgG2a control antibody (Miltenyi Biotec, Macquarie Park, NSW, Australia; cat# 130-106-546, RRID:AB_2661589); PE-conjugated mouse anti-STAT5 (pY694) antibody (BD Biosciences, Macquarie Park, NSW, Australia; cat# 612567, RRID:AB_399858); PE-conjugated rat anti-mouse CD123 (IL-3Rα) antibody (eBioscience, San Diego, CA, USA; cat# 12-1231-82, RRID:AB_465839); AF488 conjugated rat anti-mouse β IL-3 antibody (R&D Systems, Minneapolis, MN, USA; cat# FAB5492G, RRID: RRID:AB_2905558); Rat anti-mouse CD131 (mβc) antibody (BD Biosciences, cat# 740050, RRID:AB_2739817).

Techniques: Size-exclusion Chromatography, Purification, Clone Assay, Multi-Angle Light Scattering

Flow cytometric assessment of purified mAbs binding to NFS-60 cells (dark grey) natively expressing the mβc receptor. Mouse M1 cells (light grey), which do not express mβc, as well as the PE-conjugated secondary antibody and an unrelated mIgG2a isotype were used as negative controls. Cells were incubated with 200 μg/mL of each mAb, and binding is shown as a measure of the mean fluorescence intensity (MFI). Despite being isolated from the CBA biopanning method, corresponding mAbs (prefix ‘c’) generally produced a less pronounced shift indicative of a lower binding affinity for the target molecule in the native cell surface environment. Prefix ‘s’—STRE biopanning method; no prefix—binders isolated from both methods.

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of Phage Display Biopanning Strategies for the Selection of Anti-Cell Surface Receptor Antibodies

doi: 10.3390/ijms23158470

Figure Lengend Snippet: Flow cytometric assessment of purified mAbs binding to NFS-60 cells (dark grey) natively expressing the mβc receptor. Mouse M1 cells (light grey), which do not express mβc, as well as the PE-conjugated secondary antibody and an unrelated mIgG2a isotype were used as negative controls. Cells were incubated with 200 μg/mL of each mAb, and binding is shown as a measure of the mean fluorescence intensity (MFI). Despite being isolated from the CBA biopanning method, corresponding mAbs (prefix ‘c’) generally produced a less pronounced shift indicative of a lower binding affinity for the target molecule in the native cell surface environment. Prefix ‘s’—STRE biopanning method; no prefix—binders isolated from both methods.

Article Snippet: PE-conjugated rat anti-mouse CD131 (mβc) antibody (BD Pharmingen, cat# 559920, RRID: AB_397374); Mouse anti-M13 antibody (SinoBiological, Beijing, China; cat# 11973-MM05T, RRID: AB_2857926); HRP conjugated goat anti-mouse IgG antibody (BioRad, South Granville, NSW, Australia; cat# 1706516, RRID: AB_11125547); DyLight 650 conjugated goat anti-mouse IgG antibody (Abcam, Melbourne, VIC, Australia; cat# 96874, RRID:AB_10679531); Isotype mouse IgG2a control antibody (Miltenyi Biotec, Macquarie Park, NSW, Australia; cat# 130-106-546, RRID:AB_2661589); PE-conjugated mouse anti-STAT5 (pY694) antibody (BD Biosciences, Macquarie Park, NSW, Australia; cat# 612567, RRID:AB_399858); PE-conjugated rat anti-mouse CD123 (IL-3Rα) antibody (eBioscience, San Diego, CA, USA; cat# 12-1231-82, RRID:AB_465839); AF488 conjugated rat anti-mouse β IL-3 antibody (R&D Systems, Minneapolis, MN, USA; cat# FAB5492G, RRID: RRID:AB_2905558); Rat anti-mouse CD131 (mβc) antibody (BD Biosciences, cat# 740050, RRID:AB_2739817).

Techniques: Purification, Binding Assay, Expressing, Incubation, Fluorescence, Isolation, Produced